Journal: Molecular and Clinical Oncology

Article Title: A microRNA profile of pediatric glioblastoma: The role of NUCKS1 upregulation

doi: 10.3892/mco.2019.1795

Figure Lengend Snippet: Heatmap of microRNA expression across tumors (GBM1, GBM2 and GBM3) and the non-tumoral control. The color key describes the color associated with each level of expression (0–40 indicates the normalized Cq values). The heatmap was generated using R software v3.5.1 and the ‘HTqPCR’ package. GBM, glioblastoma multiforme; POOL, non-tumoral pool group.

Article Snippet: The expression of previously identified dysregulated miRNAs were determined using commercial assays (miR-137, cat. no. 001129; miR-216a, cat. no. 477976; miR-490, cat. no. 001037; miR-501-3p, cat. no. 002435; miR-521, cat. no. Hs99999903_m1; miR-525-3p, cat. no. 478995_mir; miR-873, cat. no. 478204_mir; miR-876-3p, cat. no. 002225; miR-448, cat. no. 001029; all, Thermo Fisher Scientific, Inc.; included forward and reverse primers). cDNA was synthesized using the TaqMan ® MicroRNA Reverse Transcription kit (cat. no. 4366596; Thermo Fisher Scientific, Inc.) according to the manufacturers' protocol (30 min at 16°C, 30 min at 42°C and 5 min at 85°C) with the GeneAmp ® PCR System 9700-Applied Biosystems (Thermo Fisher Scientific, Inc.). qPCR was performed using the aforementioned commercial, ready to use assays according to the protocol instructions (10 min at 95°C, 40 cycles at 15 sec at 95°C, 60 sec at 60°C) of the 7900HT Fast Real-Time PCR system (Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Generated, Software